University of North Texas

College of Arts & Sciences
Department of Biologocal Science

TO WHOM IT MAY CONCERN:

Information on Bio-S.I. Products:

This is to certify that I have been testing microbial samples from Mr.Wayne Tucker for about 14 years. Mr. Tucker brings samples to my microbiology laboratory; once a month or so for total bacterial counts and for diversity checks. Typically, samples contain about 40X 106 bacteria per ml and about 10-14 different genera. All organisms have come from healthy fertile soils. No pathogens are found and there are no genetically engineered organisms. No manure is used in any form in the preparation.

Mr. Tucker's product is known as Bio-S.L which he markets himself through his company Bio-S.L Technology. The microbial product has been consistent with little or no difference in quality and quantity over the years. Bacterial samples are plated on Tryptic Soy Agar or Nutrient Agar and grown overnight at 30°C and 3TC. Spread-Plates are checked daily for one week, colonies appearing are marked (red for day one, green for day two etc.) appropriately for identification. A picture of a week old plate from a mid-Summer sample is attached.

The Bio-S.I.™ product has an excellent record. Hundreds of pre-and ¬post- treatment photos are available for soil, water, grass and golf courses. Large areas of land have been reclaimed with treatments of Bio-S.I.™ I have tested several other microbial products over the years but found none with the consistency and diversity of Bio-S.I.™ The bacterial counts in many cases may be higher but such samples contained mostly species of the endospore-forming Bacillus and were often made using manures. No manure has ever been associated with Mr. Tucker's product.

At the outset my lab isolated and identified about 20 different organisms from a liquid sample provided by Mr. Tucker. These organisms were being used for a wide variety of purposes including improving soil performance, rejuvenating soil bacteria, removing excess phosphates and other salts from soils, diminishing odors and reclaiming lands that had been scalded with excess salts.

As testimony to the quality of Bio-S.I.™ I was invited to present a talk to the Sports Turf Management Association (STMA) at their annual national meeting in Phoenix, Arizona in February 2008. The title of the presentation was: Microbial Diversity is the Key to Healthy Soil. The talk was widely attended and well received. A number of important questions were asked and answered on the spot in a friendly atmosphere. The most important question was "What can one expect from treating poor scalded (by excess salts) soil with Bio-S.I?" The answer included: decrease or removal of salts, breakdown of recalcitrant compounds, reduction in amount of fertilizer, increase in plant growth (before and after photos). Ours was the only presentation at STMA describing live organisms being applied for bioremediation of farmland. We have been invited back to next year's national meeting in San Jose, California.

A few words about me: I received my PhD at the University of California, Davis in Microbiology with Professor John Ingraham. I spent 4 years at UC. Berkeley, three as a postdoctoral fellow with Professor John Gerhart in Molecular Biology and one year teaching Microbiology for Professors Roger Stanier and Michael Doudoroffwhere I was asked to replace Dr. George Hegeman who was on Sabbatical Leave in Paris. After Berkeley I spent 15 years on the faculty at Texas A&M University before joining the Faculty of the University of North Texas as Chairman of the Department of Biological Sciences, a position I held for 15 years. Since 1999 I have been enjoying research and teaching. I have more than 50 peer reviewed papers in a variety of journals including Nature(4) and Proceedings of the National Academy of Sciences USA(2).

My current research is studying the virulence factors of Pseudomonas aeruginosa and how virulence and pyrimidine auxotrophy are linked. My longtime research is on the regulation of pyrimidine biosynthesis in bacteria with special emphasis on the enzyme aspartate transcarbamoylase.

 

Gerard O'Donovan

Professor and Former Chairman
Dept. of Biological Sciences
University of North Texas